sumotagsequence

Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein.

The pET SUMO vector allows expression of a recombinant protein with an. N-terminal peptide containing the 6xHis tag and SUMO fusion protein. General guidelines ...

由 D Kuo 著作 · 2014 · 被引用 45 次 — A solution to this problem is the expression of proteins as fusions to solubility tags such as the SUMO protein. SUMO fusion proteins can be cleaved to remove ...

由 TR Butt 著作 · 2005 · 被引用 634 次 — Fusing a tag to the target protein provides a one-step purification procedure by passing cell extracts or supernatants over an appropriate affinity matrix.

Highly specific and active protease for efficient cleavage of SUMO tags from proteins of interest; Contains N-terminal polyhistidine tag; Useful for further ...

Sumo tag is most frequentely used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein.

SUMO-tag Protein Expression System developed by Creative BioMart utilizes SUMO to allow expression, purification, and generation of native proteins in both ...

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to ...

由 MS Team 著作 — Have you ever thought of using a solubility tag to enhance expression of your protein, considered using SUMO and then wondered, which sequence should I use?